Study on the Degradation Effect of Three Organophosphorus Hydrolase Mutant on Sarin

Organophosphorus hydrolase can effectively degrade organic phosphorus compounds such as sarin. In this study, we constructed a recombinant Bacillus subtilis mutant expressing organophosphorus hydrolase, measured the effect of on degradation rate nerve agent sarin, and selected optimal mutation scheme. Three different genes, 257L, 257Y 303T, were ligated to PMA0911 vector transferred into WB800 construct target strain successfully. The bacteria secreted protein by fermentation. enzyme sarin was determined benzidine method. screened, its enzymatic performance explored. effects three mutants hydrolysis detected. results showed that accelerated significantly. Point improve activity wild-type certain extent, laying foundation for subsequent in-depth research.